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. 2023 Aug;29(8):1243–1254. doi: 10.1261/rna.079674.123

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© 2023 Silveira d'Almeida et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 8. — RNAi Knockdown of RRP44 stabilizes both tRNA^Tyr conformers. (A,B) Northern blot hybridization performed on the extracted total RNA samples from RRP44 RNAi strain T. brucei cells subjected to Actinomycin D treatment with and without RRP44 depletion. The tRNA^Glu was used as loading control. (C–F) Linear regression calculations utilizing the amounts of mature and alternate conformer tRNA^Tyr with and without RRP44 depletion. Data were fit to a one-phase decay with R² values as indicated. All data plotted were the result of three biological replicas.