| Strain, strain background (M. musculus) |
AicdaCreERT2 |
C-A Reynaud, J-C Weill. (Institut Necker) |
|
|
| Strain, strain background (M. musculus) |
S1pr2CreERT2 BAC-transgenic |
T. Kurosaki (RIKEN-Yokohama) |
|
|
| Strain, strain background (M. musculus) |
B6.Cg-Tg(Prdm1-EYFP)1Mnz/J |
Jackson Laboratories |
|
|
| Strain, strain background (M. musculus) |
C57BL/6J |
Jackson Laboratories |
|
|
| Strain, strain background (M. musculus) |
B6.SJL |
Jackson Laboratories |
|
|
| Strain, strain background (M. musculus) |
564Igi |
Theresa Imanishi-Kari (Tufts University) |
|
|
| Strain, strain background (M. musculus) |
564Igi.1 |
This manuscript |
|
|
| Antibody |
anti-CD45.2-APC (104) (mouse monoclonal) |
Biolegend |
109814 |
1:300 |
| Antibody |
anti-CD23-BV510 (B3B4) (rat monoclonal) |
Biolegend |
101623 |
1:300 |
| Antibody |
anti-B220-PacBlue (RA3-6B2) (rat monoclonal) |
Biolegend |
103227 |
1:300 |
| Antibody |
anti-B220-PerCP/Cy5.5 (RA3-6B2) (rat monoclonal) |
Biolegend |
103234 |
1:300 |
| Antibody |
anti-GL7-PacBlue (GL7)(rat monoclonal) |
Biolegend |
144614 |
1:300 |
| Antibody |
anti-GL7-PerCP/Cy5.5 (GL7) (rat monoclonal) |
Biolegend |
144610 |
1:300 |
| Antibody |
anti-CD138-Biotin (281-2) (rat monoclonal) |
Biolegend |
142512 |
1:300 |
| Antibody |
anti-CD138-PE (281-2)(rat monoclonal) |
Biolegend |
142504 |
1:300 |
| Antibody |
anti-CD45.1-APC (A20)(mouse monoclonal) |
Biolegend |
110714 |
1:300 |
| Peptide, recombinant protein |
Streptavidin |
Biolegend |
405206 |
|
| Antibody |
anti-FCRL5-Alexa488 (sheep polyclonal) |
biotechne |
FAB6757G |
1:10 |
| Antibody |
anti-FCRL5-APC (sheep polyclonal) |
biotechne |
FAB6757A |
1:10 |
| Commercial assay, kit |
Fixable Viability Dye eFluor 780 |
Thermo Fisher |
65-0865-14 |
1:1000 |
| Commercial assay, kit |
Pan B Cell Isolation Kit II, mouse |
Miltenyi |
130-104-443 |
|
| Peptide, recombinant protein |
NP-OVA |
Biosearch |
N-5051-10 |
|
| Chemical compound, drug |
Tamoxifen |
Sigma-Aldrich |
T5648-5G |
|
| Chemical compound, drug |
Imject Alum |
Thermo Fisher Scientific |
77161 |
|
| Software, algorithm |
FlowJo |
FlowJo LLC |
|
|
| Software, algorithm |
Prism |
GraphPad |
|
|
| Software, algorithm |
R |
R Foundation |
|
4.1.2 |
| Software, algorithm |
Cellranger |
10× Genomics |
|
5.0.1 |
| Software, algorithm |
GC tree |
https://github.com/matsengrp/gctree (DeWitt et al., 2018) |
|
|
| Commercial assay, kit |
HuProt v4.0 array |
CDI Labs |
|
|
| Commercial assay, kit |
Single Cell Immune Profiling |
10× Genomics |
|
|
| Software, algorithm |
Immcantation |
https://immcantation.readthedocs.io/en/stable/
|
|
|
