Figure 5. Reduction of the Mettl3 m⁶A transferase suppresses viability and behavioral defects in Nab2 mutant females.

(A) Percent of control, Nab2^ex3, and Nab2^ex3,Mettl3^null/+ flies that eclose as viable adults (calculated as #observed/#expected). (B) Negative geotaxis of age-matched adult females of the indicated genotypes over time in seconds. (C) Survival of age-matched adult female flies of the indicated genotypes over time in days. (D) Percent of elav >Gal4 alone control, elav-Gal4;;Nab2^ex3, elav-Gal4;UAS-Mettl3-RNAi;Nab2^ex3, and elav-Gal4;UAS-Mettl3-RNAi flies that eclose as viable adults (calculated as #observed/#expected). Note that baseline Nab2^ex3 viability is elevated in the background of the elav-Gal4 transgene, and significantly suppressed by inclusion of UAS-Mettl3 RNAi. (E) Negative geotaxis assay for age-matched adult females of the indicated genotypes over time in seconds. (F) Percent of control, Nab2^ex3, Nab2^ex3,fl(2)d^2/+, or Nab2^ex3,vir^2F/+ flies that eclose as viable adults (calculated as #observed/#expected). (G) Negative geotaxis of age-matched adult females of the indicated genotypes over time in seconds. Significance values are indicated (*p<0.05, **p<0.01, ****p<0.0001).

Figure 5—figure supplement 1. Genomic PCR confirms the Nab2^ex3,Mettl3^null recombinant.

Genomic PCR of two different isolates (#10, #26) of the Mettl3^null,Nab2^ex3 double mutant chromosome balanced over TM6B. (A) and (C) show PCR products generated from each isolate using primers that flank the deletions in each gene; the normal wt product from TM6B and truncated products (Δ) are indicated. (B) and (D) show PCR products generated from each isolate using primers that lie within the deleted regions of each gene; the wt products from TM6B are indicated. Numbers of adults used for extraction of gDNA are indicated.