Fig. 1. Crystal structure of the SNX1^BAR–SNX5^BAR heterodimer and interface analysis.

a, SDS–PAGE and SEC–MALS analysis of full-length SNX1, SNX5 and SNX1–SNX5 showing the molecular weight difference between species. b, Structure of the human SNX1^BAR–SNX5^BAR heterodimer in two orthogonal views. R_c stands for radius of curvature. c–e, Close-up views of the SNX1^BAR–SNX5^BAR heterodimer interface illustrating conserved amino-acid residues (c), energetic landscape and binding hot-spot prediction (d) and electrostatic surface potential from −5 kT e⁻¹ (red) to 5 kT e⁻¹ (blue) (e). f, Electrostatic surface potential of the SNX2^BAR–SNX6^BAR heterodimerization interface generated by homology modeling. Results in a are representative of at least three independent experiments.

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