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. 2023 Jun 15;30(7):958–969. doi: 10.1038/s41594-023-01014-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) Overlap of projections of coordinates from all selected tubes. No preferred orientation can be seen. (b) Distribution of number of helical starts of all analyzed tubes. (c) Fourier shell correlation (FSC) curve for the final 3-particle map. (d) Fourier shell correlation (FSC) curve for the final 1-particle map. (e) Definition of the analyzed helix parameters (pitch, lead, lead angle, channel width) and relations to neighboring particles (a, b, alpha, beta). (f) Tube radius (mean +/− SD) related to number of helical starts N. Mean and standard deviation have been computed on the available number of filaments detected for each N: 10 filaments for N = 1, 57 filaments for N = 2, 49 filaments for N = 3 and 65 filaments for N = 4. (g) Distances between the centers of the particles related to N. (h) Helical pitch and channel width related to N. Helical pitch is presented as mean values +/− SD. (i) Angle alpha related to N. (j) Angle beta related to N. (k) Cryo-ET data processing workflow. (l) Average of subtomograms from individual particles displaying local resolution (Å) coloured from highest resolution (red) to lowest resolution (dark blue).