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. 2023 Jun 15;30(7):958–969. doi: 10.1038/s41594-023-01014-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — (a) Summary of Kds between the CI-MPR bipartite sorting motif (amino acids 2347-2375) titrated into the SNX1-SAH^3A:SNX5-SAH^3A mutant or the SNX1- BT*:SNX5- BT* mutant. Values are the mean and standard deviation (SD) from two independent experiments. (b) Representative ITC experiments for the binding of the previous SAH3A and BT* mutants. Top panels show the raw data and bottom panels represent the integrated and normalized data fit with a 1:1 binding model. (c) Circular dichroism (CD) spectra of SNX1^WT-SNX5^WT, the SNX1-SAH^3A:SNX5-SAH3A mutant, and the SNX1- BT*:SNX5- BT* mutant. (d) Liposome flotation assay of SNX1^WT-SNX5^WT, the SNX1-SAH^3A:SNX5-SAH3A mutant, and the SNX1-BT*:SNX5-BT* mutant. The liposome composition was: DOPC/DOPE/DOPS/PtdIns(3)P/Liss Rhod-PE 45:28:20:5:2 molar ratio. Data are representative of two (a-c), or three (d) independent experiments.