Fig. 4.

Transcriptome analysis of overexpressed miR-526b-5p in trophoblasts. A Volcano diagram of differentially expressed genes. The abscissa is the log2FoldChange, and the ordinate is −log10 (P-value). The two vertical dashed lines are the 2-fold differential expression threshold. The dotted line indicates the threshold of P-value=0.05. The red dots are the upregulated genes, the blue dots are the downregulated genes, and the grey dots indicate nonsignificant differentially expressed genes. B Bar chart of GO enrichment analysis. The abscissa is the term of Go level2, and the ordinate is the enriched −log10 (P-value) of each term. GO classification was carried out according to molecular function (MF), biological process (BP), and cell component (CC), and the top 10 GO terms with the minimum P-value, namely, the most significant enrichment, were selected for display in each GO classification. C The expression of CDK2, EGFR, P53, and MMP1 was verified by quantitative RT-qPCR. The results are presented as the mean ± SD (n = 6). *P < 0.05, **P < 0.01, and ***P < 0.001 (miR-526b-5p, miR-526b-5p precursor expression vector; miR-NC, pCDH513B-1 empty vector)