Table 1.
Repeatability tests (= “within-run” precision). Repeatability was assessed under 6 different conditions. The last two conditions (conditions 5 and 6) used a buffered medium with stable pH in order to limit the matrix effect. For conditions 1 to 4, the medium was used in microdroplets under an oil overlay and incubated for 24 hours. Ten dishes were prepared for each condition. The same conditions were measured in succession in the same series
| Condition | Matrix | Incubation | Number of measurements | Mean | SD | CV (%) |
|---|---|---|---|---|---|---|
| 1 | Global Total HSA® |
K system G210 + incubator CO2 level 1 (8%) (lab 1) |
10 | 7.200 | 0.015 | 0.21 |
| 2 | Global Total HSA® |
K system G210 + incubator CO2 level 2 (5%) (lab 1) |
10 | 7.373 | 0.012 | 0.17 |
| 3 | Global Total HSA® | EmbryoScope incubator CO2 level 1 (8.5%) (lab 2) | 10 | 7.240 | 0.016 | 0.22 |
| 4 | Global Total HSA® | EmbryoScope incubator CO2 level 2 (5.5%) (lab 2) | 10 | 7.394 | 0.009 | 0.12 |
| 5 | Medium with stable pH level 1 (Eurotrol BGEM level 1) | No incubation (lab 1) | 9 | 7.013 | 0.005 | 0.07 |
| 6 | Medium with stable pH level 1 (Eurotrol BGEM level 3) | No incubation (lab 1) | 10 | 7.724 | 0.008 | 0.11 |