Table 1.
Primers sequences used in current study
| Genes | Sequence (5’-3’) | |
|---|---|---|
|
OPN (mouse) |
Sense | GACCACAGGACGACGATG |
| Antisense | TGGAACTTGCTTGACTATCGA | |
|
Runx2 (mouse) |
Sense | GACTGTGGTTACCGTCATGGC |
| Antisense | ACTTGGTTTTTCATAACAGCGGA | |
|
Osterix (mouse) |
Sense | CTGCGGAAAGGAGGCACAAAGAAG |
| Antisense | GGGTTAAGGGGAGCAAAGTCAGAT | |
|
Osteocalcin (mouse) |
Sense | ACGGTATCACTATTTAGGACCTGTG |
| Antisense | ACTTTATTTTGGAGCTGCTGTGAC | |
|
Col1a1 (mouse) |
Sense | ATGTTCAGCTTTGTGGACCTC |
| Antisense | CAGAAAGCACAGCACTCGC | |
|
COX-1 (mouse) |
Sense | ATTGCCCTCCCCTCTCTACGCA |
| Antisense | CGTAGCTTCAGTATCATTGGTGCCC | |
|
β-actin (mouse) |
Sense | CCATGTTCCAAAACCATTCC |
| Antisense | GGGCAACCTTCCCAATAAAT | |
| GAPDH | Sense | TATGTCGTGGAGTCTACTGGT |
| (mouse) | Antisense | GAGTTGTCATATTTCTCGTGG |
|
ALP (rat) |
Sense | GGGTCAAGGCCAACTACAAGA |
| Antisense | CACTGGTCTAATCGAGCAGC | |
| Osteocalcin (rat) | Sense | TCTCTGCTCACTCTGCTGG |
| Antisense | GTGGTGCCATAGATGCGCT | |
|
TRAP (rat) |
Sense | AATTGCCTACTCCAAGATCTCCAA |
| Antisense | GCGGAACTTTGAAACGCAAA | |
|
GAPDH (rat) |
Sense | TATCACTCTACCCACGGCAAG |
| Antisense | ATACTCAGCACCAGCATCACC |