Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| Chicken (EMD-29552) (PDB 8FY4) | Human (EMD-29551) (PDB 8FY3) | |
|---|---|---|
| Data collection and processing | SIMPLE/CryoSPARC/RELION | |
| Magnification | 165,000 | |
| Voltage (kV) | 300 | |
| Electron exposure (e–/Å2) | 51.2 to 58.4 | |
| Defocus range (μm) | −0.25 to –2.5 | |
| Pixel size (Å) | 0.723 | 0.693 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 17,295,060 | 15,740,546 |
| Final particle images (no.) | 760,888 | 387,942 |
| Map resolution (Å) | 2.57 | 2.88 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | PHENIX/COOT | |
| Model resolution (Å) | 3.0 | 3.0 |
| FSC Threshold | 0.5 | 0.5 |
| Map sharpening B factor (Å2) | −98.9 | −114.4 |
| Model composition | ||
| Non-hydrogen atoms | 7786 | 8948 |
| Protein residues | 988 | 1123 |
| B factors (Å2) | 106.9 | 72.5 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.002 | 0.003 |
| Bond angles (°) | 0.448 | 0.492 |
| Validation | ||
| MolProbity score | 1.48 | 2.06 |
| Clashscore | 5.54 | 7.8 |
| Poor rotamers (%) | 1.75 | 3.0 |
| Ramachandran plot | ||
| Favored (%) | 98.3 | 96.0 |
| Allowed (%) | 1.6 | 4.0 |
| Disallowed (%) | 0.1 | 0.0 |