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. 2023 Jul 17;14(7):442. doi: 10.1038/s41419-023-05947-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A WGCNA analysis of transcriptome of PT subtypes of mice. Each leaf (vertical line) in the dendrogram corresponds to a gene. B Module-trait relationship based on WGCNA analysis of PT subtypes of mice. C The network of module 1 of the WGCNA analysis. D Violin plot of AGT in PT subtypes of db/m and db/db mice. E The kidneys of db/m mice and db/db mice were collected and staining by CODEX Multiplexed Tissue Staining. PT (AQP1, green), PT^AQP4+ (AQP4, yellow), AGT (pink), DAPI (blue). Scale bars, 180 μm. ▲ (triple stain of AQP1, AQP4 and AGT). F KEGG pathway analysis of module 1 of the WGCNA analysis. G Violin plot of CP in PT subtypes of db/m and db/db mice. H Urinary CP/Cr levels in db/m (n = 6) and db/db mice (n = 6). I The relationship between urinary CP/Cr and albuminuria, NGAL/Cr and Kim-1/Cr in mice. The level of CP, NGAL and Kim-1 in the urine measured using ELISA. Levels of urinary albumin and Cr assessed using the immunoturbidimetric assay and the enzymatic method respectively. J KEGG pathway analysis of module 6 of the WGCNA analysis. K The kidneys of db/m mice and db/db mice were collected and staining by CODEX multiplexed tissue staining. PT (AQP1, red), GPX4 (yellow), and DAPI (blue). Scale bars, 120 μm. L The kidneys of a normal individual and a type 2 diabetes (T2D) patient were collected and staining by Immunohistochemistry (IHC) stain. PT (AQP1, green) and GPX4 (brown). Scale bars, 50 μm. ▲ (double stain of GPX4 and AQP1). The bar graph represents the mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test, and p-value of correlation was analyzed by Spearman analysis.