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. 2023 Jul 17;14:4271. doi: 10.1038/s41467-023-39769-8

Fig. 3. PD-1H co-localizes with podosome in OCL.

Fig. 3

A WT OCLs were stained with Acti-stain 488 phalloidin (for F-actin) (green), PE-anti-PD-1H (red) and DAPI (blue) and subjected to confocal microscopy. Left panel: cartoon of podosome (green) patterns during the different stages of OCL development. From top to bottom: clusters, rings, and sealing zone-like belt. Scale bar, 100 μm. The experiment was performed three times with similar observations. B BMCs from WT or Pd-1h-/- mice were cultured in OCL differentiation medium without or with MMP-13 stained with Acti-stain 488 phalloidin (for F-actin) (green), PE-anti-PD-1H (red) and DAPI (blue). Podosome clusters, ring or sealing zone-like belt-forming OCL numbers per field were quantified from randomly imaged fields (n = 6/group). Mean ± SD. *P ≤ 0.05, **P ≤ 0.01 by one-way ANOVA. Scale bar, 50 μm.