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. 2023 Jul 17;9:252. doi: 10.1038/s41420-023-01538-3

Fig. 2. Fgr expression in RIPF lung senescent cells mediates induction of profibrotic gene products in target cells separated in transwell culture.

Fig. 2

Control and RIPF tdTOMp16+ mouse lungs (0 Gy, and 18 Gy, 130 days) were enzymatically digested to form single-cell suspension, and RIPF lung cells were FACS sorted for irradiated senescent (tdTOM+) and irradiated non-senescent (tdTOM−) cells. A Induction of Fgr in control, and thoracic-irradiated lung cells. B Upregulation of profibrotic genes (TGF-β, Col1a1, Col3, Col4, Col5, and Col6) in target C57 control cells by thoracic-irradiated lung cells relative to non-irradiated lung cells and FACS purified tdTOMp+ lung senescent cells relative to control, non-senescent tdTOM− cells. The induction of profibrotic genes is abrogated by TL02-59 treatment (10 nM) for 10 days. (n = 3, P values were calculated by non-parametric t test).