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. 2023 Jul 17;9:252. doi: 10.1038/s41420-023-01538-3

Fig. 3. Fgr inhibitor TL02-59 reduces levels of profibrotic gene products and p16 in thoracic-irradiated mouse lungs.

Fig. 3

Thoracic-irradiated (18 Gy) C57BL/6 mice were treated with either vehicle or Fgr inhibitor TL02-59 (10 mg/kg) by oral gavage at day 50 for 4 weeks (3 times a week, alternative days). At Day 130 after irradiation, the mice were sacrificed and one lobe of the lungs were collected for qPCR assessment. A Expression of fibrotic genes (Collagen 1, Collagen 3, Collagen 4, TGF-β, and CTGF) were assessed by qPCR. B Expression of Fgr and senescent biomarkers p16, p21 were assessed by qPCR. C Immunofluorescence staining of the lungs showing Fgr (Green), p16 (Red), and colocalized (yellow) lung cells from control, thoracic-irradiated, and TL02-59 treated. Quantification of Fgr and p16-positive cells are shown on the right. (n = 4–10 mice, P values were obtained by non-parametric t test, IF quantification was done using cells/equivalent areas (n = 3), p values were calculated by ANOVA, Turkey’s multiple comparison test).