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. 2023 Jul 17;14(7):445. doi: 10.1038/s41419-023-05944-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B The expression of talin1 and talin2 were detected by western blot in cells of BCKDK overexpression or knockdown. C IF assay showed the colocalization of talin1 and BCKDK in MCF-7 cells. Cells were stained for BCKDK (red) and talin1 (green). Scale bar, 50 μm. D, E BCKDK was coimmunoprecipitated with talin1 in MCF-7 and MDA-MB-231 cells. F Exogenous Talin1 coimmunoprecipitated with BCKDK in HEK293T cells. G The ubiquitination of talin1 was examined by western blot in HEK293T cells.