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. 2023 Jul 17;14(7):439. doi: 10.1038/s41419-023-05966-y

Fig. 5. SLC7A11 plays an important role in the growth and invasion of osteosarcoma cells and is the key downstream gene of MLX.

Fig. 5

A WB analysis of MLX and SLC7A11 in 143B (left) and SJSA1 (right) upon MLX knockdown. B–E The measurement of Cysteine, GSH, ROS and lipid peroxidation level upon overexpressing SLC7A11 in MLX-silenced 143B cells. Data are represented as mean ± SD, n = 3. Unpaired t-test was used. F, G Relative growth curves of 143B (F) and SJSA1 (G) cells stably transduced with nontargeting scrambled control shRNA (shCont) or two SLC7A11 shRNAs (shSLC7A11#1 and shSLC7A11#2). Data are represented as mean ± SD, n = 3. Unpaired one-way ANOVA test followed by Dunnett’s test was used. Cell invasion upon knockdown of shMLX and/or overexpression SLC7A11 in 143B (H) and SJSA1 (I) cells. Data are represented as mean ± SD, n = 3. Unpaired t-test was used. ***: P ≤ 0.001, **: P ≤ 0.01, *: P ≤ 0.05, n.s.: not significant.