Table 2.
Calculated resolution values for the investigated dihydroxylated vitamin D3 metabolites
| 1,25(OH)2D3 and 24,25(OH)2D3—resolution (Rs) | ||||
|---|---|---|---|---|
| Compounds | Chromatographic conditions (column/mobile phase) | |||
| C-18/H2O-MeOH | C-18/H2O-ACN | F5/H2O-MeOH | F5/H2O-ACN | |
| 1,25(OH)2D3 and 24,25(OH)2D3 | 2.1 | 0.8 | 5.3 | 0.3 |
| 1,25(OH)2D3-PTAD and 24,25(OH)2D3-PTAD | 4.6 | 1.5 | 3.7 | 0.6 |
| 1,25(OH)2D3-PTAD-Ac and 24,25(OH)2D3-PTAD-Ac | 1.9 | 3.2 | 0.8 | 2.1 |
| 1,25(OH)2D3-Amplifex and 24,25(OH)2D3-Amplifex | 3.7 | 0.4 | 2.7 | 0.5 |
| 1,25(OH)2D3-FMP-TS and 24,25(OH)2D3-FMP-TS | 5.3 | 1.7 | 4.4 | 1.5 |
| 1,25(OH)2D3-INC and 24,25(OH)2D3-INC | 7.9 | 5.4 | 1.6 | 2.4 |
| 1,25(OH)2D3-PyrNO and 24,25(OH)2D3-PyrNO | 5.1 | 0.0 | 3.8 | 0.3 |
| 1,25(OH)2D3-DMEQ-TAD and 24,25(OH)2D3-DMEQ-TAD | 3.1 | 3.6 |
10.9 (Fig. 3) |
0.1 |