Table 3.
Calculated resolution values for mono-hydroxylated vitamin D3 metabolites
| 3β-25(OH)D3 and 3α-25(OH)D3—resolution (Rs) | ||||
|---|---|---|---|---|
| Compounds | Chromatographic conditions (column/mobile phase) | |||
| C-18/H2O-MeOH | C-18/H2O-ACN | F5/H2O-MeOH | F5/H2O-ACN | |
|
3β-25(OH)D3 and 3α-25(OH)D3 |
0.0 | 0.0 | 1.6 | 0.5 |
| 3β-25(OH)D3-PTAD and 3α-25(OH)D3-PTAD | 0.4 | 0.4 | 0.3 | 0.1 |
| 3β-25(OH)D3-PTAD-Ac and 3α-25(OH)D3-PTAD-Ac | 2.9 |
3.1 (Fig. 4) |
1.1 | 0.6 |
| 3β-25(OH)D3-Amplifex and 3α-25(OH)D3-Amplifex | 0.0 | 0.2 | 0.0 | 0.0 |
| 3β-25(OH)D3-FMP-TS and 3α-25(OH)D3-FMP-TS | 0.4 | 0.4 | 1.2 | 1.1 |
|
3β-25(OH)D3-INC and 3α-25(OH)D3-INC |
0.0 | 0.5 | 2.3 | 1.5 |
| 3β-25(OH)D3-PyrNO and 3α-25(OH)D3-PyrNO | 1.9 | 2.5 |
3.5 (Fig. 5) |
0.3 |
| 3β-25(OH)D3-DMEQ-TAD and 3α-25(OH)D3-DMEQ-TAD | 0.0 | 0.0 | 1.0 | 0.0 |