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. 2023 Jun 7;415(19):4731–4740. doi: 10.1007/s00216-023-04766-9

Table 1.

Experimental partition coefficients K (here: between lower and upper phase, KL/U) from shake flask experiments and calculated corrected elution volumes (CEV) for flow rate combinations A (mobile phase 2 mL/min and stationary phase 4 mL/min) and B (mobile phase 4 mL/min and stationary phase 2 mL/min) in co-current CCC (ccCCC) and C with conventional CCC (CCC) with a mobile phase flow rate of 2 mL/min. CEV for ccCCC (Eq. 4) and CCC (CEV=K·Sf) [26] were calculated with a coil volume of 236 mL and an Sf value of 88% in tail-to-head mode

Lipid Shake flask KL/U CEV A [%]
ccCCC
CEV B [%]
ccCCC
CEV C [%]
CCC
Tripalmitin (1) 0.02 23.1 3.3 1.8
Cholesteryl stearate (2) 0.02 23.1 3.3 1.8
Squalene (3) 0.04 25.9 4.2 3.5
Dipalmitoylglycerol (4) 0.35 52.9 16.7 30.8
Dihydrolanosterol (5) 0.37 54.0 17.4 32.6
α-Tocopherol (6) 0.51 60.4 22.0 44.9
β-Sitosterol (7) 0.66 65.5 26.3 58.1
Campesterol (8) 0.73 67.5 28.2 64.2
Cholesterol (9) 0.74 67.7 28.4 65.1
Stearic acid (10) 1.20 76.5 38.5 105.6
Elaidic acid (11) 1.45 79.5 42.9 127.6
Palmitic acid (12) 1.50 80.0 43.7 132.0