Table 1.
Experimental partition coefficients K (here: between lower and upper phase, KL/U) from shake flask experiments and calculated corrected elution volumes (CEV) for flow rate combinations A (mobile phase 2 mL/min and stationary phase 4 mL/min) and B (mobile phase 4 mL/min and stationary phase 2 mL/min) in co-current CCC (ccCCC) and C with conventional CCC (CCC) with a mobile phase flow rate of 2 mL/min. CEV for ccCCC (Eq. 4) and CCC () [26] were calculated with a coil volume of 236 mL and an Sf value of 88% in tail-to-head mode
| Lipid | Shake flask KL/U | CEV A [%] ccCCC |
CEV B [%] ccCCC |
CEV C [%] CCC |
|---|---|---|---|---|
| Tripalmitin (1) | 0.02 | 23.1 | 3.3 | 1.8 |
| Cholesteryl stearate (2) | 0.02 | 23.1 | 3.3 | 1.8 |
| Squalene (3) | 0.04 | 25.9 | 4.2 | 3.5 |
| Dipalmitoylglycerol (4) | 0.35 | 52.9 | 16.7 | 30.8 |
| Dihydrolanosterol (5) | 0.37 | 54.0 | 17.4 | 32.6 |
| α-Tocopherol (6) | 0.51 | 60.4 | 22.0 | 44.9 |
| β-Sitosterol (7) | 0.66 | 65.5 | 26.3 | 58.1 |
| Campesterol (8) | 0.73 | 67.5 | 28.2 | 64.2 |
| Cholesterol (9) | 0.74 | 67.7 | 28.4 | 65.1 |
| Stearic acid (10) | 1.20 | 76.5 | 38.5 | 105.6 |
| Elaidic acid (11) | 1.45 | 79.5 | 42.9 | 127.6 |
| Palmitic acid (12) | 1.50 | 80.0 | 43.7 | 132.0 |