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. 2023 Apr 20;22(7):e13850. doi: 10.1111/acel.13850

FIGURE 5.

FIGURE 5

GCV treatment alters tissue damping and reverses CS‐induced airspace enlargement in the lungs of young p16‐3MR mice. p16‐3MR mice were subjected to chronic (3 months) CS exposure, followed by 5‐day treatment with GCV (25 mg/kg body weight) or PBS (control). (a) Lung function parameters of PBS/GCV treated air/CS‐exposed p16‐3MR mice were determined using SCIREQ's flexiVent system. Data are shown as mean ± SEM (n = 6–7/groups). p‐value calculated as per one‐way ANOVA for multiple comparisons. The lung tissues from air‐ and CS‐exposed mice were harvested and H&E stained. (b) Representative images of n = 4/group were provided and the (c) quantified counts were plotted as mean ± SEM. Scale bar: 100 μm (20×). Changes in the observed Lm values for young and old mice was analyzed and plotted. Scale bar: 100 μm (20×). (d) Representative images of n = 2/group were provided and the (e) quantified counts were plotted as mean ± SEM. Arrows denote airspace enlargement in the lung on CS‐treatment. SE: *p < 0.05, **p < 0.01, ***p < 0.001; as per one‐way ANOVA for multiple comparisons. p16‐3MR mice were subjected to chronic (6 months) ETS exposure, followed by 5‐day treatment with GCV (25 mg/kg body weight) or PBS (control). The OCT sections from air‐ and ETS‐exposed mouse lungs were H&E stained. (f) Representative images of n = 3–4/group were provided and the (g) quantified counts (bottom) were plotted as mean ± SEM. Scale bar: 100 μm (20×).