Table 2.

Amino acid analyses of proteins recovered using the ultrasonic-assisted alkaline method with different power settings.

Total amino acid	0 W	150 W	300 W	450 W
Asp	8.37 ± 0.02^a	9.09 ± 0.13^b	9.11 ± 0.08^b	9.37 ± 0.16^a
Thr	2.75 ± 0.04^b	2.93 ± 0.06^a	2.94 ± 0.01^a	2.95 ± 0.12^a
Ser	3.77 ± 0.06^b	4.14 ± 0.09^a	4.19 ± 0.07^a	4.22 ± 0.07^a
Glu	14.73 ± 0.04^c	17.06 ± 0.05^b	17.14 ± 0.15^b	17.55 ± 0.11^a
Gly	3.02 ± 0.07^b	3.41 ± 0.14^a	3.44 ± 0.03^a	3.46 ± 0.13^a
Ala	2.95 ± 0.01^b	3.28 ± 0.08^a	3.30 ± 0.10^a	3.33 ± 0.05^a
Cys	0.70 ± 0.08^b	0.92 ± 0.11^a	0.84 ± 0.04^ab	0.91 ± 0.08^a
Val	3.26 ± 0.02^b	3.37 ± 0.02^a	3.38 ± 0.09^a	3.38 ± 0.06^a
Met	0.85 ± 0.04^b	0.96 ± 0.07^ab	1.03 ± 0.14^a	1.04 ± 0.09^a
Trp	–	–	–	–
Ile	3.30 ± 0.12^b	3.44 ± 0.09^ab	3.47 ± 0.06^a	3.54 ± 0.03^a
Leu	5.83 ± 0.07^b	6.06 ± 0.16^ab	6.10 ± 0.08^a	6.20 ± 0.19^a
Tyr	2.59 ± 0.05^b	2.79 ± 0.09^a	2.84 ± 0.04^a	2.88 ± 0.01^a
Phe	3.88 ± 0.05^a	3.59 ± 0.07^b	3.63 ± 0.12^b	3.67 ± 0.13^b
Lys	4.39 ± 0.07^a	4.17 ± 0.04^c	4.22 ± 0.07^bc	4.30 ± 0.05^ab
His	1.82 ± 0.03^b	1.93 ± 0.07^ab	1.95 ± 0.12^ab	2.00 ± 0.08^a
Arg	5.70 ± 0.08^b	5.69 ± 0.08^b	5.77 ± 0.06^ab	5.87 ± 0.06^a
Pro EAA Hydrophobic Hydrophilic	3.38 ± 0.05^a 26.09 26.47 9.81	3.00 ± 0.15^b 26.45 27.12 10.77	2.93 ± 0.06^b 26.72 27.28 10.81	2.99 ± 0.16^b 27.08 27.61 10.95

Different letters in the same column indicate significant differences (P < 0.05).