FIG. 5.

(A) Western blot for PilA expression from 5 × 10⁶ whole cells. (B) Detection of extracellular pili (PilA) from 2 × 10⁸ cells. The pili were sheared off the cells by passage through a 25-gauge 3.5-inch needle as previously described (59). The strains are DK1622 (WT), DK8613 (ΔL), and DK8615 (ΔQ).