TABLE 1.
M. xanthus strains and plasmids
| Strain or plasmid | Genotype or relevant properties | Phenotype | Construction | Reference |
|---|---|---|---|---|
| M. xanthus | ||||
| DK101 | pilQ1 (sglA1) | A+ S− | M. xanthus FB | 15 |
| DK320 | aglB1 pilQ1 | A− S− | UV on DK101 | 21 |
| DK1217 | aglB1 | A− S+ | Mx8 (YS) × DK320→screen S+ | 22 |
| DK1241 | aglR4 pilQ1241 | A− S− | UV | 20a |
| DK1242 | cglB pilQ1242 | A− S− | UV | 20a |
| DK1243 | agl-12 pilQ1243 | A− S− | UV | 22 |
| DK1247 | aglK2 pilQ1247 | A− S− | UV | 20a |
| DK1287 | agl1 pilQ1287 | A− S− | UV | 20a |
| DK1291 | aglK1 pilQ1291 | A− S− | UV | 20a |
| DK1600 | pilG or pilH | A+ S− | Spontaneous; YS (MD2) | 56 |
| DK1609 | agl pilQ1609 | A− S− | ICR-191 | 37a |
| DK1622 | Wild type | A+ S+ | Mx8 (YS) × DK1217→screen A+ | 37a |
| DK1627 | cglC1 pilQ1627 | A− S− | ICR-191 | 37a |
| DK1633 | cglC1 pilQ1633 | A− S− | ICR-191 | 37a |
| DK1636 | cglB2 pilQ1636 | A− S− | ICR-191 | 37a |
| DK1675 | cglB2 pilQ1675 | A− S− | ICR-191 | 37a |
| DK1687 | agl pilQ1687 | A− S− | 37a | |
| DK2136 | aglB1 pilQ2136 | A− S− | ICR-191 | 37a |
| DK2140 | agl pilQ2140 | A− S− | ICR-191 | 37a |
| DK2148 | aglB1 pilQ2148 | A− S− | ICR-191 | 37a |
| DK2149 | aglB1 pilQ2149 | A− S− | ICR-191 | 37a |
| DK2150 | aglB1 pilQ2150 | A− S− | ICR-191 | 37a |
| DK2159 | aglB1 pilQ2159 | A− S− | ICR-191 | 37a |
| DK2167 | cglB2 pilQ10 rif-100 | A− S− | Spontaneous; YNS | 37a |
| DK2227 | cglB2 pilQΩ2227 | A− S− | 37a | |
| DK4293 | Ω4401 | A+ S+ | 26 | |
| DK4414 | Ω4414 | A+ S+ | 26 | |
| DK8610 | Ω3188 pilQ+ | A+ S+ | Mx4 (DK10389) × DK101→select Kmr; screen S+ | |
| DK8611 | Ω3188 pilQ1 | A+ S− | Mx4 (DK10389) × DK101→select Kmr; screen S− | |
| DK8612 | Wild type | A+ S+ | pDW140 × DK101→select Kmr; select Galr; screen Kms; screen by Southern blotting | |
| DK8613 | ΔorfL | A+ S+ | pDW146 × DK1622→select Kmr; select Galr; screen Kms; screen by Southern blotting | |
| DK8614 | aglB1 ΔorfL | A− S+ | pDW146 × DK1217→select Kmr; select Galr; screen Kms; screen by Southern blotting | |
| DK8615 | ΔpilQ | A+ S− | pDW131 × DK1622→select Kmr; select Galr; screen Kms; screen by Southern blotting | |
| DK8616 | aglB1 ΔpilQ | A− S− | pDW131 × DK1217→select Kmr; select Galr; screen Kms; screen by Southern blotting | |
| DK8617 | ΔpilQ Ω4401 | A+ S− | Mx4 (DK4293) × DK8615→select Kmr | |
| DK8618 | ΔpilQ Ω4414 | A+ S− | Mx4 (DK4414) × DK8615→select Kmr | |
| DK10389 | Ω3188 pilQ+ | A+ S+ | Mx4 (DK3188) × DK1622→select Kmr | |
| Plasmids | ||||
| pBluescript SK | Cloning vector | Apr | Stratagene (La Jolla, Calif.) | |
| pBGS18 | Cloning vector | Kmr | 51 | |
| pKG-2 | nptII galK | Apr Kmr | Kmr Gals cassette | 52 |
| pDW79 | Ω3188; pil region | Apr Kmr | 19-kb (Ω3188) ClaI-ClaI from DK10389 cloned in pBluescript (ClaI) | |
| pDW81 | pilQ fragment | Kmr | 2.1-kb MluI-MluI from pDW79 cloned in pBGS18 (SmaI) | |
| pDW83 | pilQ fragment | Kmr | Exonuclease III deletion of pDW81, leaving 1.0 kb | |
| pDW85 | pilQ fragment | Kmr | Exonuclease III deletion of pDW81, leaving 0.75 kb | |
| pDW92 | pil region | Kmr | 6.2-kb HindIII-KpnI from pDW79 cloned in pBGS18 (HindIII-KpnI) | |
| pDW94 | pil region | Kmr | 4.5-kb HindIII-KpnI from pDW79 cloned in pBGS18 (HindIII-KpnI) | |
| pDW105 | pil region | Apr | 6.2-kb HindIII-KpnI from pDW79 cloned in pBluescript (HindIII-KpnI) | |
| pDW131 | ΔpilQ orfL galK | Kmr Apr | PCR-generated ΔpilQ; nptII galK cassette cloned in pDW105 (BamHI-EcoRI) | |
| pDW137 | orfL | Apr | 2.9-kb BamHI-EcoRI from pDW105 in pBluescript | |
| pDW140 | pilQ fragment, galK | Kmr Apr | 2.0-kb SphI from pDW81 cloned in pBluescript (pDW96 EcoRV); nptII galK cassette cloned in EcoRI | |
| pDW146 | ΔorfL galK | Kmr Apr | PCR-generated ΔorfL cloned in pDW137 (EcoNI-EcoRI); nptII galK cloned in EcoRV | |
| pDW139 | orfL | Kmr | 5.0-kb KpnI-MscI (blunted and ligated) deletion of pDW92 | |
| pDW167 | pilQ orfL | Kmr | 2.3-kb KpnI-NotI (blunted and ligated) deletion of pDW92 | |
| pDW168 | pil region | Kmr | 11-kb HindIII from pDW79 cloned in pBGS18 (HindIII) | |
| pDW169 | pil region | Kmr | 3.3-kb PshAI-NotI from pDW79 cloned in pBGS18 (SmaI) | |
| pDW188 | pilQ orfL | Kmr | 2.1-kb KpnI-PstI (blunted and ligated) deletion of pDW92 |