Fig. 5.

The Human respirovirus 1 (HRV1) fusion (F) protein chain A amino acid sequences investigated in this study. The amino acid residue numbers used for constructing HRV1 F protein three-dimensional (3D) structure are highlighted in yellow. The amino acid residues of neutralising antibodies (NT-Ab) binding sites and mutation are highlighted in orange and light green, respectively. The black and green line boxes in the sequences are the conformational epitope and linear epitope sites, respectively. Blue line boxes in the sequence indicate negative selection sites detected by all four methods (single-likelihood ancestor counting, fixed effects likelihood, internal fixed-effects likelihood, and fast unconstrained Bayesian approximation).