TABLE 1.
Bacterial strains used and growth on ManNAc
| Strain | Growth on ManNAca | Relevant genotypeb | Reference or source |
|---|---|---|---|
| IBPC5321 | +P | thi-1 argG6 argE3 his-4 mtl-1 xyl-5 rpsL ΔlacX74 mlc-1 | 31 |
| IBPC900 | +P | IBPC5321 mlc::Tc | |
| IBPC904 | +++ | IBPC5321 ama-4 | |
| IBPC905 | +++ | IBPC900 ama-5 | |
| IBPC915 | + | IBPC900 mlc+nth1::Km | |
| JM101 | + | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/supE thi Δ(lac-proAB) | |
| IBPC1012 | +P | JM101 mlc::Tc | 30 |
| IBPC1016 | +++ | IBPC1012 ama-6 | |
| Ymc | + | supF lacY | |
| IBPC1013 | +P | Ymc mlc::Tc | |
| IBPC1017 | +++ | IPBC1013 ama-7 | |
| IBPC1047 | + | IBPC1017 mlc+ nth1::Km | |
| IBPC1008 | NT | Ymc nth1::Km | P1(PK2242) × Ymc |
| PK2242 | NT | HfrC Δ1646(dgsA-manA) ptsG tonA22 nth1::Km | P. Kuempel 30 |
| MC4100 | + | araD139 Δ(argF-lac)U169 flbB3501 deoC1 relA1 rbsR rpsL150 ptsF25 | |
| KD622 | +P | MC4100 φ(malK-lacZ)1113 (λplacMu50) mlc::Tn10Km | 6 |
| IBPC1018 | +++ | KD622 ama-8 | |
| IBPC707 | − | IBPC5321 manXYZ::Tn9 | IBPC5321 × P1(NK6702) |
| IBPC567 | − | IBPC5321 ptsM8 | 31 |
| IBPC546 | − | IBPC5321 nagB::Km | 31 |
| IBPC531 | − | IBPC5321 nagA::Cm | 31 |
| IBPC529C | +P | IBPC5321 nagC::Cm | 31 |
| IBPC542 | +P | IBPC5321 nagE::Km | 31 |
| IBPC720 | +P | IBPC5321 ptsG22 zcf229::Tn10 | IBPC5321 × P1(TP2506) |
| IBPC750 | +P | IBPC5321 glmS::Tc | |
| IBPC1000 | +P | IBPC720 mlc::Tn10kan | |
| IBPC1001 | +++ | IBPC1000 ama-1 | |
| IBPC1011 | +P | IBPC1001 Tn10cam11 | IBPC1001 × P1(λNK1324) |
| IBPC1021 | +++ | IBPC1016 Tn10cam11 (ama-6) | IBPC1016 × P1(IBPC1011) |
| IBPC1022 | +P | IBPC1012Tn10cam11 (ama+) | IBPC1016 × P1(IBPC1011) |
| IBPC1023 | +++ | IBPC1017 Tn10cam11 (ama-7) | IBPC1017 × P1(IBPC1011) |
| IBPC1024 | +P | IBPC1013 Tn10cam11 (ama+) | IBPC1017 × P1(IBPC1011) |
| IBPC1027 | +P | JM101 mlc::Tc Tn10kan27 Cms | IBPC1022 × P1(λNK1316) |
| IBPC1028 | +P | Ymc mlc::Tc Tn10kan28c Cms | IBPC1024 × P1(λNK1316) |
| IBPC1029 | +P | Ymc mlc::Tc Tn10kan29 Cms | IBPC1024 × P1(λNK1316) |
| IBPC1033 | +P | JM101 mlc::Tc Tn10kan33 Cms | IBPC1022 × P1(λNK1316) |
| IBPC1059 | +++ | JM101 mlc::Tc ama-7 Tn10cam11 | IBPC1012 × P1(IBPC1023) |
| TP2506 | NT | lacZ ptsG22 glk-7 rha-4 rpsL223 zcf229::Tn10 | 7 |
| NK6702 | NT | man-6::Tn9 IN(rrnD-rrnE)1 | E. coli Genetics Stock Center |
a
−, no growth after 1 week, +, very slow background growth (tiny colonies after 3 to 4 days); +P, papillae (formation of isolated large colonies during slow background growth); +++, good growth on ManNAc; NT, not tested. Cms, chloramphenicol sensitive.
b
Sequencing showed that Tn10cam11 is gltD::Tn10cam and that Tn10kan27 and -28 are yhcA::Tn10kan.