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Phages and plasmids used
| Phage or plasmid | Genotype | Reference |
|---|---|---|
| Phages | ||
| λNK1316 | λ b522 cI857 Pam80 nin5 (mini-Tn10kan/Ptac-ATSa transposase) | 14 |
| λNK1324 | λ b522 cI857 Pam80 nin5 (mini-Tn10cam/Ptac-ATS-transposase) | 14 |
| λRS/EB | λRS45/nagB-lacZ | 34 |
| Plasmids | ||
| pSX600 | nanAT yhcJIH | 19 |
| pTZ(Nan4-5) | plac yhcJ | This work |
| pTZ(Nan4-6) | plac yhcJIH | This work |
| pTZ(Nan1-3) | plac yhcK | This work |
| pBR(Nan4-5) | yhcJ | This work |
| pBR(Nan4-6) | yhcJIH | This work |
| pBR(Nan1-3) | yhcK | This work |
ATS, altered target specificity (14).
