TABLE 3.
Effects of ManNAc and sialic acid on expression of nagB-lacZ fusiona
| Strain | Genotype | β-Galactosidase activity (Miller units)c
|
|||
|---|---|---|---|---|---|
| ManNAcb | Sialic acid | GlcNAc | Glc | ||
| JM101 | Wild type | NT | 1,830 | 3,030 | 68 |
| 1012 | mlc | NT | 1,770 | 3,140 | 69 |
| 1016 | ama-6 mlc | 3,890 | 1,660 | 3,400 | 68 |
| 1059 | ama-7 mlc | 4,300 | 1,330 | 3,400 | 59 |
a
Bacteria were lysogenized with λRS/EB carrying a nagB-lacZ fusion.
b
Bacteria were grown in minimal MOPS medium with the carbon sources indicated at 30°C.
c
Measured at four points during exponential growth. Values are means of at least two independent cultures. NT, not tested.