TABLE 4.
Growth of mlc ama strains on ManNAc in the presence of different nan region plasmidsa
| Plasmid | Growthb
|
||||
|---|---|---|---|---|---|
| JM101 (wild type) | 1012 (mlc) | 1016 (mlc ama-6) | 1013 (mlc) | 1017 (mlc ama-7) | |
| pSX600 | + | ++ | +++ | ++ | NT |
| pBR/Nan4-6 | + | +++ | NT | +++ | NT |
| pBR/Nan4-5 | + | +++ | NT | +++ | NT |
| pBR/Nan1-3 | − | − | − | NT | − |
| pBR322 | + | +P | +++ | +P | +++ |
a
Bacteria were transformed with the plasmids indicated by selecting for ampicillin resistance on LB plates and then tested for growth on minimal ManNAc plates. Plates used for testing for repression by pBR(Nan1-3) contained 0.1 mg of ampicillin ml−1.
b
−, no growth; +, slow background growth; ++, medium uniform growth; +P, papillae (formation of isolated large colonies during slow background growth); +++, good growth on ManNAc; NT, not tested.