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SDS-PAGE analysis of protein fractions. Proteins were separated on an SDS–12% polyacrylamide gel and stained with Coomassie brilliant blue. Lanes: 1, molecular weight markers; 2, crude extract of E. coli JM109 harboring pBluescript II KS(+) (5 μg of protein); 3, crude extract of E. coli JM109 harboring pDS15 (5 μg of protein); 4, PI fraction (5 μg of protein); 5, HQ fraction (2 μg of protein); 6, HP2 fraction (2 μg of protein). Molecular masses are given on the left.
