TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. paucimobilis | ||
| SYK-6 | Wild type; Nalr Smr | 12 |
| DLI | Mutant derivative of SYK-6; Kmr gene insertion mutant of ligI; Nalr Smr Kmr | This study |
| P. putida PpY1100 | Nalr Smr | 12 |
| E. coli JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15] | 39 |
| Plasmids | ||
| pUC18 and pUC19 | Cloning vectors; Apr | 39 |
| pBluescript II KS(+) | Cloning vector; Apr | 35 |
| pK19mobsacB | oriT sacB Kmr | 31 |
| pKT230 | Broad-host-range vector; Kmr | 2 |
| pHN139F | pUC18 with a 10.5-kb EcoRI fragment of SYK-6 carrying ligAB and ligI | This study |
| pHN139R | pUC18 carrying the same fragment as pHN139F in the opposite direction | This study |
| pSS50F | KS(+) with a 5.0-kb SmaI fragment carrying ligI of pHN139 | This study |
| pSS50R | KS(+) carrying the same fragment as pSS50F in the opposite direction | This study |
| pDS15 | Deletion derivative of pSS50R carrying ligIa | This study |
| pSS32F, pSS14F, pSS55F, pSS73F, pSS32R, pSS14R, pSS55R, pSS73R | Deletion derivatives of pHN139F and pHN139Ra | This study |
| pUC1923 | pUC19 with a 2.3-kb PstI-SmaI fragment carrying ligI | This study |
| pUC1923K | pUC1923 with insertion of the Kmr gene of pUC4K into a StuI site | This study |
| pLID1 | pK19mobsacB with a 3.6-kb EcoRI-SmaI fragment of pUC1923K | This study |
| pVA01 | pKT230 with a 10.5-kb EcoRI fragment carrying ligAB and ligI | 12 |
| pVAD4 | Deletion plasmid of pVA01; SalI fragments in the middle of the insert were deleteda | 22 |
a
See Fig. 2.