TABLE 2.
Purification of PDC hydrolase from E. coli harboring pDS15
| Fraction | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Fold |
|---|---|---|---|---|---|
| Crude extract | 200 | 673 | 3.40 | 100 | 1.0 |
| PI | 14.0 | 158 | 11.3 | 23 | 3.3 |
| HQ | 2.00 | 104 | 52.7 | 15 | 16 |
| HP2 | 0.50 | 66.0 | 128 | 10 | 38 |
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