TABLE 2.
Susceptibility testing of recombinant P. aeruginosa PAO strainsa
| Ambler class | Minimal inhibitory concentrations (μg/mL) |
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain (β-lactamase produced) | β-lactamase spectrum | CAZ | CZA | FEP | FEP-TAN | FEP-ENM | MEM | MVB | MER-NAC | MER-NAC 1:1 | IPM | I-R | ATM | ATM-AVI | NAC | FEP-ZID | FEP-ZID 1:1 | ZID | |
| P. aeruginosa (none) | -b | - | 1 | 1 | 1 | 1 | 1 | 0.5 | 0.25 | 0.25 | 0.25 | 0.5 | 0.25 | 4 | 2 | >32 | ≤0.125 | 1 | 4 |
| P. aeruginosa OXY-2 | ESBL | A | 4 | 1 | >256 | 1 | 2 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.25 | 64 | 2 | >32 | 2 | 2 | 8 |
| P. aeruginosa TEM-1 | Narrow | A | 2 | 1 | 2 | 1 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.25 | 4 | 4 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa TEM-3 | Narrow | A | 8 | 1 | 16 | 2 | 1 | 0.5 | 0.5 | 0.25 | 0.5 | 0.5 | 0.25 | 4 | 4 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa CTX-M-3 | ESBL | A | 16 | 4 | 256 | 2 | 1 | 0.5 | 0.25 | 0.5 | 0.5 | 0.5 | 0.5 | >256 | 2 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa CTX-M-15 | ESBL | A | 16 | 1 | >256 | 2 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 64 | 4 | >32 | 2 | 4 | 8 |
| P. aeruginosa CTX-M-33 | Carba | A | 16 | 2 | >256 | 2 | 1 | 2 | 0.5 | 0.5 | 0.5 | 2 | 0.5 | 128 | 4 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa GES-1 | ESBL | A | 32 | 1 | 8 | 1 | 2 | 0.5 | 0.25 | 0.25 | 0.5 | 0.5 | 0.5 | 4 | 2 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa GES-2 | Carba | A | 8 | 1 | 8 | 2 | 2 | 1 | 0.5 | 0.5 | 1 | 1 | 0.25 | 16 | 4 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa GES-5 | Carba | A | 16 | 2 | 2 | 1 | 2 | 4 | 1 | 0.5 | 1 | 2 | 1 | 4 | 2 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa GES-6 | Carba | A | 64 | 2 | 2 | 1 | 2 | 8 | 2 | 2 | 4 | 4 | 1 | 4 | 2 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa BEL-1 | ESBL | A | 32 | 2 | 16 | 2 | 8 | 0.5 | 0.25 | 0.25 | 0.25 | 0.5 | 0.5 | 64 | 2 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa BEL-2 | ESBL | A | 128 | 4 | 2 | 1 | 2 | 0.5 | 0.5 | 0.5 | 0.25 | 0.5 | 0.5 | 32 | 4 | >32 | 0.5 | 2 | 8 |
| P. aeruginosa SHV-2b | ESBL | A | 128 | 4 | >256 | 4 | 64 | 0.5 | 0.25 | 0.25 | 0.5 | 1 | 0.25 | 64 | 2 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa SHV-1 | Narrow | A | 64 | 2 | 128 | 2 | 32 | 0.5 | 0.25 | 0.25 | 0.5 | 1 | 0.25 | 8 | 2 | >32 | 4 | 4 | 8 |
| P. aeruginosa SHV-12 | ESBL | A | >256 | 4 | >256 | 1 | 64 | 0.5 | 0.25 | 0.25 | 0.25 | 1 | 0.5 | >256 | 8 | >32 | 0.25 | 4 | 8 |
| P. aeruginosa VEB-1 | ESBL | A | >256 | 64 | >256 | 8 | 32 | 0.5 | 0.5 | 0.25 | 0.25 | 1 | 0.5 | >256 | 16 | >32 | 128 | 8 | 8 |
| P. aeruginosa FRI-1 | Carba | A | 2 | 1 | 2 | 1 | 1 | 1 | 0.25 | 0.25 | 4 | 2 | 0.25 | 16 | 2 | >32 | 0.5 | 2 | 8 |
| P. aeruginosa PER-1 | ESBL | A | >256 | 32 | >256 | 4 | >128 | 0.5 | 0.25 | 0.5 | 0.5 | 1 | 0.25 | >256 | 16 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa PER-2 | ESBL | A | 128 | 4 | 32 | 1 | 1 | 0.5 | 0.25 | 0.5 | 0.25 | 1 | 0.25 | >256 | 8 | >32 | 4 | 4 | 8 |
| P. aeruginosa PER-6 | ESBL | A | >256 | 32 | >256 | 4 | 1 | 1 | 0.5 | 0.25 | 0.25 | 0.5 | 0.5 | >256 | 32 | >32 | 4 | 4 | 8 |
| P. aeruginosa PER-7 | ESBL | A | >256 | 32 | >256 | 8 | >128 | 1 | 0.25 | 0.25 | 0.5 | 1 | 0.5 | >256 | 32 | >32 | 8 | 8 | 8 |
| P. aeruginosa KPC-2 | Carba | A | 128 | 4 | >256 | 4 | >128 | 128 | 16 | 4 | 4 | 64 | 1 | >256 | 8 | >32 | >128 | 8 | 8 |
| P. aeruginosa KPC-3 | Carba | A | >256 | 8 | >256 | 4 | 128 | 128 | 16 | 4 | 4 | 64 | 1 | >256 | 8 | >32 | >128 | 8 | 8 |
| P. aeruginosa KPC-41 | Carba | A | >256 | >128 | >256 | 32 | >128 | 32 | 8 | 4 | 4 | 16 | 2 | >256 | 32 | >32 | >128 | 8 | 8 |
| P. aeruginosa SME | Carba | A | 8 | 1 | 4 | 1 | 1 | 64 | 2 | 4 | 4 | 64 | 1 | >256 | 4 | >32 | 2 | 2 | 8 |
| P. aeruginosa NMC-A | Carba | A | 4 | 1 | 4 | 1 | 1 | 32 | 2 | 2 | 2 | 32 | 1 | 128 | 4 | >32 | 2 | 2 | 8 |
| P. aeruginosa IMI-1 | Carba | A | 2 | 1 | 8 | 8 | 8 | 16 | 1 | 1 | 4 | 16 | 1 | 128 | 4 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa VIM-1 | Carba | B | >256 | >128 | >256 | 128 | >128 | 64 | 64 | 64 | >32 | 32 | 32 | 4 | 4 | >32 | >128 | 8 | 8 |
| P. aeruginosa VIM-2 | Carba | B | 128 | 128 | 128 | 4 | 128 | 32 | 32 | 32 | 32 | 16 | 16 | 4 | 4 | >32 | 128 | 8 | 8 |
| P. aeruginosa AIM-1 | Carba | B | 64 | 64 | 16 | 16 | 16 | 32 | 32 | 32 | 32 | 16 | 16 | 4 | 2 | >32 | 0.5 | 4 | 8 |
| P. aeruginosa GIM-1 | Carba | B | 64 | 64 | 8 | 4 | 8 | 16 | 16 | 16 | 16 | 4 | 4 | 4 | 4 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa DIM-1 | Carba | B | 256 | 128 | 2 | 2 | 2 | 16 | 16 | 16 | 16 | 16 | 16 | 4 | 4 | >32 | ≤0.125 | 2 | 8 |
| P. aeruginosa NDM-1 | Carba | B | >256 | >128 | >256 | 64 | >128 | 64 | 64 | 64 | >32 | 16 | 16 | 4 | 4 | >32 | >128 | 8 | 8 |
| P. aeruginosa NDM-5 | Carba | B | >256 | >128 | >256 | 64 | >128 | 32 | 32 | 32 | 32 | 16 | 16 | 4 | 4 | >32 | >128 | 8 | 8 |
| P. aeruginosa NDM-7 | Carba | B | >256 | >128 | >256 | 64 | >128 | 64 | 64 | 64 | >32 | 16 | 16 | 4 | 4 | >32 | >128 | 8 | 8 |
| P. aeruginosa NDM-9 | Carba | B | >256 | >128 | >256 | >128 | >128 | 64 | 64 | 64 | >32 | 16 | 16 | 4 | 4 | >32 | >128 | 8 | 8 |
| P. aeruginosa IMP-1 | Carba | B | >256 | >128 | >256 | >128 | >128 | 64 | 64 | 64 | >32 | 16 | 16 | 4 | 4 | >32 | 128 | 8 | 8 |
| P. aeruginosa SPM-1 | Carba | B | >256 | 128 | >256 | 16 | >128 | 32 | 32 | 32 | 32 | 4 | 4 | 4 | 4 | >32 | ≤0.125 | 4 | 8 |
| P. aeruginosa CMY-2 | Case | C | >256 | 128 | 128 | 16 | 64 | 1 | 0.5 | 0.5 | 1 | 1 | 1 | 256 | 16 | >32 | 32 | 8 | 8 |
| P. aeruginosa CMY-42 | Case | C | >256 | 64 | 128 | 2 | 64 | 1 | 0.5 | 0.5 | 1 | 1 | 1 | 256 | 16 | >32 | 32 | 8 | 8 |
| P. aeruginosa DHA-1 | Case | C | 128 | 1 | 2 | 2 | 1 | 0.5 | 0.25 | 0.25 | 0.25 | 1 | 0.5 | 16 | 4 | >32 | 0.5 | 2 | 8 |
| P. aeruginosa OXA-1 | Narrow | D | 1 | 1 | 32 | 32 | 4 | 0.5 | 0.5 | 0.25 | 0.25 | 0.5 | 0.5 | 4 | 2 | >32 | 2 | 4 | 8 |
| P. aeruginosa OXA-18 | Carba | D | >256 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 4 | 4 | 64 | 4 | >32 | 0.25 | 1 | 8 |
| P. aeruginosa OXA-23 | Carba | D | 2 | 1 | 32 | 16 | 16 | 8 | 8 | 8 | 8 | 4 | 4 | 4 | 2 | >32 | 4 | 4 | 8 |
| P. aeruginosa OXA-48 | Carba | D | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 4 | 2 | >32 | 1 | 1 | 8 |
| P. aeruginosa OXA-58 | Carba | D | 1 | 1 | 1 | 1 | 1 | 4 | 4 | 4 | 4 | 2 | 2 | 4 | 2 | >32 | ≤0.125 | 1 | 8 |
| P. aeruginosa OXA-181 | Carba | D | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 4 | 2 | >32 | 0.25 | 1 | 8 |
| P. aeruginosa OXA-427 | Carba | D | >256 | 128 | 64 | 4 | 4 | 4 | 4 | 4 | 4 | 2 | 2 | >256 | 32 | >32 | ≤0.125 | 4 | 8 |
a
Narrow, narrow-spectrum; Case, Cephalosporinase; ESBL, extended-spectrum β-lactamase; Carba, Carbapenemase; CAZ, ceftazidime; CZA, ceftazidime-avibactam; FEP, cefepime; FEP-ZID, cefepime/zidebactam; FEP-TAN, cefepime/taniborbactam; FEP-ENM, cefepime/enmetazobactam; IPM, imipenem; I-R, imipenem/relebactam; MEM, meropenem; MVB, meropenem-vaborbactam; MER-NAC, meropenem/nacubactam; ATM, aztreonam; ATM-AVI, aztreonam/avibactam. The MIC values indicated in bold are those corresponding to a categorization of resistance. The shaded MIC values are those that correspond to a significantly elevated MIC value, compared to wild type P. aeruginosa PAO1, but are not categorized as resistant, according to EUCAST (12).
b
-, none.