FIG 1.
Novel C. trachomatis type III secreted effector proteins are secreted using Y. pseudotuberculosis as a surrogate host. Y. pseudotuberculosis was transformed with each C. trachomatis gene of interest, and transformants were grown in secretion-inducing (− Ca2+) or secretion-inhibiting (+ Ca2+) medium. Pellets (P) and supernatants (S) were separated by centrifugation and normalized by optical density at 600 nm. Samples were resolved by SDS-PAGE expression, and immunoblots were probed with anti-FLAG antibodies. Data are representative of three independent experiments.