FIG 2.
ypfP mutant cells demonstrate reduced anaerobic proliferation. (A) CFU of WT or ypfP mutant bacteria generated after incubation under aerobic (+O2) or anaerobic (−O2) conditions were enumerated after 24 h at 37°C. Potassium nitrate (KNO3; 100 mM) was used to induced anaerobic respiration. Data are means from three independent experiments performed in triplicate. Error bars represent one standard deviation from the mean. Statistical significance was determined by a two-way ANOVA with Tukey’s multiple-comparison test. ****, P < 0.0001. (B) The WT and the ypfP mutant were subcultured 1:100 from an overnight culture and grown anaerobically at 37°C. Growth was measured at the indicated time points by monitoring OD600. The experiment was performed in triplicate. Error bars represent standard deviations from the mean.