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Presence of intratumoural microbiome in hepatocellular carcinoma and its costaining with immune cells. (A) Fluorescence in situ hybridisation (FISH) analysis of bacterial 16S rRNA in a tumour sample stained with EUB388 probe (red) and DAPI (blue). (B, C) Costaining of bacterial 16S rRNA with immune cells. CD8+ T cells are marked by pink (B), and macrophages are marked by green (C). The arrows indicate signals of intratumoural bacteria within CD8+ cells. Scale bar = 100 μm.
