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. 2023 Jul 4;4:1126012. doi: 10.3389/falgy.2023.1126012

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© 2023 Newton, Zhang, Donohue, Unnithan, Cedrone, Xu, Vermilya, Malys, Clogston and Dobrovolskaia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AmBisome nor Feraheme modify cell population and activation statuses in immunophenotyping panel #1. PBMC were treated with positive controls (LPS/PHA-M or PMA-Ionomycin), negative controls (unstimulated/untreated, Dextrose Control, PBS Control), 1 µg/ml AmBisome or 1 mg/ml Feraheme for 24 h. Dextrose was a control for AmBisome and PBS was a control for Feraheme. (A) The top heat map indicates the average stimulation index (SI) of percentage of treated cell populations (AmBisome or Feraheme) as compared to unstimulated (or untreated) populations for each of the three human donors—each donor had two replicates for each condition. Cell population percentages were defined as percentage of live cells. The middle heat map is a representative heat map of the average percentage of cells in each of the different activation quadrants for CD19, CD8, and CD4 cells from Donor O4Q7 (2 replicates per condition). The bottom heat map is a representative heat map from Donor O4Q7 (2 replicates per condition) indicating the average stimulation index of the geometric mean fluorescence intensity (gMFI) of the activation markers in each cell population as compared to the unstimulated cells. (B) Tables representing the average stimulation indices of the different cell populations as compared to the unstimulated PBMC for each of the three donors (2 replicates per condition for each of the 3 donors). The top table indicates changes in cell populations. The next three tables indicate fold changes in percentages of activated cells in CD19, CD8, and CD4 populations and the last three indicate the fold changes for gMFI of activated cells in CD19, CD8, and CD4 populations.