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Purification steps of DABA acetyltransferase from H. elongata OUT30018
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U mg−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude extracta | 1,100 | 130 | 0.12 | 1.0 | 100 |
| Ammonium sulfate | 850 | 120 | 0.14 | 1.2 | 96 |
| HIC | 60 | 75 | 1.3 | 11 | 58 |
| Blue Sepharose I | 0.90 | 10 | 11 | 94 | 8 |
| Blue Sepharose II | 0.05 | 2.5 | 50 | 420 | 2 |
Cells from a 5-liter culture were used.
