TABLE 4.

Purification steps of ectoine synthase from H. elongata OUT30018

Purification step	Total protein (mg)	Total activity (U)	Sp act (U mg−1)	Purification (fold)	Yield (%)
Crude extracta	912	45.6	0.050	1.0	100
Ammonium sulfate	684	40.6	0.059	1.2	89
HIC	6.4	20.5	3.2	64	45
HAb	0.81	11.4	14	280	25
Gel filtration	0.50	8.0	16	320	18

^aCells from a 5-liter culture were used. 

^bHA column chromatography.