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. 2023 Jul 4;5:1177586. doi: 10.3389/ftox.2023.1177586

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Copyright © 2023 Perez-Diaz, Hoffman, Clements, Cruickshank, Doherty, Ebner, Elloway, Fu, Kelsall, Millar, Saib, Scott, Woods and Hutter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterisation of TK6 cell line during gradual adaptation and long term incubation in chemically defined serum replacement (CDSR) supplemented media. Cell viability (A), cell count (B), and cell size (C) were measured every cell passage and pooled every three passages and expressed as mean (±SD). Dotted lines show the lowest values of cell viability (A), growth (C), and cell diameter (D) of cells growing in 10% (v/v) FBS-supplemented medium. Significance between conditions was assessed using a one-way ANOVA. The data are presented as mean ± SD.