Table 1.
Oligonucleotides used in this study.
| Primer name | Sequence (5′–3′, restriction sites in bold) | Description |
|---|---|---|
| BbLptD F | GCGGCTAGCCAGACTATAGATGATGAAAATTC | Antibody production (nucleotides 88–873) |
| BbLptD R | GCGCTCGAGACTATCTCCCGGTCTGAAAAAA | |
| BbLptDNT-S F | GCGCATATGCAGACTATAGATGATGAAAATTC | Cloning the N-terminal domain of bblptD into S tag of pACYCDuet-1 (nucleotides 90–813) |
| BbLptDNT-S R | GCGGGTACCTATGGCATTTAAAAACCCAAAATC | |
| BbLptA-S F | GCGCATATGACACAAATAGAATCCAGCCTTA | Cloning bblptA into S tag of pACYCDuet-1 (nucleotides 60–end) |
| BbLptA-S R | GCGGGTACCTTTTTTTTCTTCAGAAGCATCATT | |
| BbLptA-His F | GCGGGATCCTACACAAATAGAATCCAGCCTTA | Cloning bblptA into 6xHis tag of pACYCDuet-1 (nucleotides 60–end) |
| BbLptA-His R | GCGGAGCTCTTATTTTTTTTCTTCAGAAGCATC | |
| BbLptC-His F | GCGGGATCCTTCTAGTAGATCTGATGTGGC | Cloning bblptC into 6xHis tag of pACYCDuet-1 (nucleotides 69–end) |
| BbLptC-His R | GCGGAGCTCTCAATTCATGATTCCTTCAACTC | |
| OspC-S F | GCGCATATGGGGAAAGATGGGAATACATCT | Cloning ospC into S tag of pACYCDuet-1 (nucleotides 69–end) |
| OspC-S R | GCGGGTACCAGGTTTTTTTGGACTTTCTGC | |
| BbLptD up mutant F | GCGGGTACCACAGAAATTAGGGAGCTTTTTG | Cloning 600 base pairs upstream of bblptD |
| BbLptD up mutant R | GCGCTCGAGCTATAAAAGATTTTTTAAAAACATTCC | Cloning 600 base pairs upstream of bblptD |
| BbLptD down mutant F | GCGGGATCCCATATGCGAGAATTCCTATACAGGAATG | Cloning 600 base pairs downstream of bblptD start |
| BbLptDdown mutant R | GCGTCTAGAGGTAACATCATTGTCTATCTTTT | Cloning 600 base pairs downstream of bblptD start |