FIG. 4.

Genome organization of the P. aeruginosa phpA region. The region contained within the insert of pSW109 is indicated. Additional 5′ sequence information was obtained from the P. aeruginosa Genome Sequencing Project (15 June 1998 release). ORF280 displays 34% amino acid identity to an ORF of unknown function in M. tuberculosis. The orientation of Tn5-B50 enables transcription of ORF280 while at the same time inactivating phpA and possibly exerting a polar effect on holC. Restriction endonuclease sites correspond to those shown in Fig. 2. Abbreviations: B, BamHI; E, EcoRI; L, SalI; S, SphI; X, XhoI; H, HindIII.