Skip to main content
. Author manuscript; available in PMC: 2023 Jul 18.
Published in final edited form as: ACS Synth Biol. 2019 Jun 10;8(6):1314–1324. doi: 10.1021/acssynbio.8b00528

Figure 3. NIRW light and cAMP-dependent gene expression in E. coli expressing engineered ACs.

Figure 3.

(a) The AC assay is based on the cAMP-CRP-dependent lacZ expression in the E. coli cya mutant12. CRP is a cAMP-dependent transcription activator46. (b) Images of AC-dependent β-galactosidase activity. E. coli BL21[DE3] cya carrying plasmids for expression of the NIRW-AC, pET23::AC, and heme oxygenase, pT7-ho110. Strains were grown on LB agar containing X-Gal (40 μM) and IPTG (25 μM), either in the dark or in the red (660 nm) light.