Skip to main content
. Author manuscript; available in PMC: 2023 Jul 18.
Published in final edited form as: ACS Synth Biol. 2019 Jun 10;8(6):1314–1324. doi: 10.1021/acssynbio.8b00528

Figure 4. Photochemical characterization of NIRW-AC variants in vitro.

Figure 4.

(a) Kinetics of cAMP accumulation by the purified IlaC*, IlaD9, IlaM4 and IlaM5 proteins in the dark and light, at 22 oC and 37 oC. Results of one experiment representative of three independent protein purifications are shown. (b) The UV-vis absorption spectra of IlaM5. (c) Kinetics of the dark recovery of IlaM5 from the lit Pfr state. Plotted are temporally changes in absorbance at 750 nm. Shown are averages from three technical replicates performed using one protein preparation.