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. Author manuscript; available in PMC: 2023 Jul 18.
Published in final edited form as: ACS Synth Biol. 2019 Jun 10;8(6):1314–1324. doi: 10.1021/acssynbio.8b00528

Figure 5. Light-activated cAMP synthesis by NIRW-ACs expressed in HEK293 cells.

Figure 5.

(a) cAMP pathway in mammalian cells. (b) Adeno-associated viral construct encoding the NIRW-AC (either IlaC* or IlaM5), heme oxygenase BphO, and green fluorescent protein eGFP, used as transfection control from the neuronal hSyn promoter. (c) Comparison of NIRW-AC activities of IlaC* and IlaM5 expressed in HEK293 cells. Cell cultures expressing IlaC* or IlaM5 were irradiated with red (660 nm) light and changes in the cAMP levels were monitored by measuring secreted luciferase MetLuc expressed from the cAMP-dependent promoter. Shown are average data from three biological replicates, each performed in duplicate, ± standard deviation.