TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference(s) |
|---|---|---|
| E. coli strains | ||
| DH5α | F−endA1 hsdR17 (rK−) supE44 thi-1 λ− recA1 deoR gyrA96 relA1 Δ(argF-lacZYA)U169 φ80dlacZΔM15 | Bethesda Research Laboratories |
| S17-1::λpir | Host of pUT-Tn5(pfm)CmKm | 66 |
| X. campestris pv. campestris strains | ||
| 17 | Wild-type strain isolated in Taiwan, Apr | 68 |
| 17R | Spontaneous Rifr derivative of strain 17 | This study |
| Mutants of 17R isolated by Tn5(pfm)CmKm mutagenesis | ||
| AS20A(ura-1) | Auxotrophic for uracil | This study |
| G56A(cys-1) | Auxotrophic for cysteine | This study |
| A48A(trp-1) | Auxotrophic for tryptophan | This study |
| AL89A(ade-1) | Auxotrophic for adenosine | This study |
| AM39A(gua-1) | Auxotrophic for guanosine | This study |
| A32A(gln-1) | Auxotrophic for glutamine | This study |
| BC3A(gly-1) | Auxotrophic for glycine | This study |
| BG49A(thr-1) | Auxotrophic for threonine | This study |
| T72A(his-1) | Auxotrophic for histidine | This study |
| BL81E | Nonmucoid mutant, complemented by pP2402B containing rfbCDAB-pmi gene cluster | This study |
| BB15E | Low-mucoid mutant | This study |
| BG44E | Nonmucoid mutant, defective in UDP-glucose dehydrogenase | 33 |
| BM23E | Low-mucoid mutant | This study |
| AY61E | Low-mucoid mutant | This study |
| G76E | Nonmucoid mutant, defective in UDP-glucose pyrophosphorylase | 58, 59 |
| AH53E | Nonmucoid mutant, complemented by pP3DA01B containing gum gene cluster | This study |
| AU56E | Low-mucoid mutant | This study |
| XKB | Uncharacterized | This study |
| Plasmids | ||
| pUT-Tn5(pfm)CmKm | R6K derivative carrying mini-Tn5 for pulsed-field mapping, Cmr Kmr | 66 |
| pNEB193 | pUC19 derivative carrying unique sites for AscI, PacI, and PmeI | New England Biolabs |
| pX727 | pFY7 containing a 0.5-kb Sau3A1 fragment from the tdh gene of X. campestris pv. campestris 17 | 44 |
| pPPE3 | pNEB193 containing a 0.6-kb PstI fragment from PE of X. campestris pv. campestris 17 chromosome | This study |
| pW2Y2 | Cosmid clone derived from pCP13 carrying an insert of 38 kb from X. campestris pv. campestris 17 chromosome with unique sites for SwaI and PacI | This study |
| pPWDR3 | pNEB193 containing a 1.0-kb PstI fragment from WD of X. campestris pv. campestris 17 chromosome | This study |
| pREF | pUC18 derivative containing a 0.3-kb PstI fragment cloned from the overlapping region of PA and WC of X. campestris pv. campestris 17 chromosome | This study |
| pRRNSM3 | pUC18 derivative containing the 0.7-kb SmaI-PstI fragment internal to the X. campestris pv. campestris 17 23S rrn spanning the I-CeuI site | This study |
| pP2402B | Cosmid clone derived from pLAFR1 containing a ca. 24-kb Sau3A1 partial fragment, including the rfbCDAB-pmi gene cluster | 51, 52 |
| pP3DA01B | Cosmid clone derived from pLAFR1 containing a ca. 25-kb EcoRI fragment, including the gum gene cluster | 69 |
| pSD701 | Cosmid clone derived from pLAFR1 containing a 5.3-kb EcoRI fragment conferring UDP-glucose dehydrogenase | 33 |
| pEK6 | pUC18 derivative containing a 3.0-kb EcoRI-KpnI fragment conferring UDP-glucose pyrophosphorylase | 58, 59 |