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. 2023 Jun 5;3(7):796–812. doi: 10.1038/s43587-023-00436-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a. Western blot analysis of MCU, TOM20, and MICU1 protein. N = 5–15 mice, error bars reflect SEM; p-value determined by Welch’s t-test, two-tailed. b. Quantitative-PCR of Mcu and its regulatory subunits. N = 3 mice. Error bars reflect SEM; p-value determined by Welch’s t-test, two-tailed. c. Resolution of MCU complex in non-reducing conditions and immunoblotting for MCU and MICU1, N = 1 mouse. d. Pulsed mitochondrial Ca²⁺ uptake. N = 15 biological replicates.

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