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. Author manuscript; available in PMC: 2023 Jul 19.
Published in final edited form as: Cell Rep. 2022 Jul 26;40(4):111095. doi: 10.1016/j.celrep.2022.111095

Figure 4. SHP099 sensitivity correlates with NF1 levels in neuroblastoma.

Figure 4.

(A) Graph represents SHP099 sensitivity (area under the curve [AUC]) with respect to NF1 transcript levels obtained from R2 genomic (Sanger) database (http://hgserver1.amc.nl) in 31 available NB cells lines from the HTS. Pearson correlation analysis value: r = 0.4982; p = 0.0043.

(B) Immunoblot analysis of SHP099-sensitive (red) and SHP099-resistant (black) NB cell lines probed for NF1, SHP2, and other indicated antibodies. GAPDH was used as a loading control.

(C) Immunoblot analysis of NF1 and pERK expression in NF1-silenced NB cell lines insensitive to SHP099. Scrambled oligonucleotide was used as an siRNA control (siCtrl), and GAPDH was used as a loading control.

(D) Graph indicates relative levels of pERK in NF1-silenced NB cell lines quantified from (C).

See also Figures S8-S11.