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. 2023 Feb 21;48(9):1328–1337. doi: 10.1038/s41386-023-01551-1

Fig. 4. Chronic corticosterone treatment impairs in vivo dopamine transporter (DAT) function in the dorsomedial striatum (DMS) of male mice.

Fig. 4

A Experimental timeline for viral injection, fiber optic implant, pellet implantation, and open field behavior. B Representative image of dLight1.3b viral spread and fiber optic implantation site (outlined in dashed white line). Scale bar equals 500 micrometers. C Representative activity traces of male mice (Placebo, top; CORT, bottom) during the ten-minute baseline period (‘Baseline’) and the last ten minutes of recorded activity after injection of the DAT inhibitor GBR12909 (20 mg/kg, i.p., ‘DATi’). D Velocity of Placebo- (N = 10) and CORT- (N = 9) treated male mice, in averaged five-minute bins, before and after injection with the DAT inhibitor, GBR12909 (20 mg/kg, i.p.; injection time indicated by vertical dashed line). Two-Way ANOVA, main effect of treatment *p < 0.05, trending effect of time p = 0.06. E Change in dLight1.3b area-under-the-curve (AUC) relative to the minute average of the ten-minute baseline period prior to injection with the DAT inhibitor, GBR12909 (20 mg/kg, i.p.; injection time indicated by vertical dashed line) in male mice. Two-Way ANOVA, main effect of treatment *p < 0.05, main effect of time p < 0.01, main effect of treatment x time interaction p < 0.0001. Placebo N = 5, CORT N = 6. F Representative activity traces of female mice (Placebo, top; CORT, bottom) during the ten-minute baseline period (‘Baseline’) and the last ten minutes of recorded activity after injection of the DAT inhibitor GBR12909 (20 mg/kg, i.p., ‘DATi’). G Velocity of Placebo- (N = 6) and CORT- (N = 8) treated female mice, in averaged five-minute bins, before and after injection with the DAT inhibitor, GBR12909 (20 mg/kg, i.p.; injection time indicated by vertical dashed line). Two-Way ANOVA, main effect of time p < 0.001. H Change in dLight1.3b AUC relative to the average of the ten-minute baseline period prior to injection with the DAT inhibitor, GBR12909 (20 mg/kg, i.p.; injection time indicated by vertical dashed line) in female mice. Two-Way ANOVA, main effect of time p < 0.01. Placebo N = 5, CORT N = 6. Data presented as mean ± SEM.