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. 2023 May 23;48(9):1309–1317. doi: 10.1038/s41386-023-01608-1

Fig. 3. CIN D2R upregulation in NAc does not alter timing.

Fig. 3

A Schematic representation of the temporal discrimination task. In each session, a single response on one of two lever options is rewarded based on the duration of the sample auditory stimulus. Two tone durations are presented in each session. B Mean proportion of correct responses on the corresponding lever in 2-s tone trials (short, left) or 8-s tone trials (long, right) across blocks of 2 sessions was not altered by D2R upregulation. A 3-way ANOVA showed no virus main effect, F (1, 65) = 0.005903, p = 0.9390, and no significant virus x tone duration x session block (F(4, 65) = 0.9819, p = 0.4237) or virus x session block (F (4, 65) = 0.05092, p = 0.9950) or virus x tone duration (F (1, 65) = 0.2230, p = 0.6383) interactions; n = 8 EGFP (7 females, 1 male), 7 D2 (6 females, 1 male). C The duration of tones was proportionally increased to 6 s (short, left) and 24 s (long, right). While there was a significant main effect of session block on discrimination of tone durations (F (7, 104) = 21.52, p < 0.0001), no significant main effect of virus (F (1, 104) = 1.890, p = 0.1721), or virus x tone duration x session block (F (7, 104) = 0.2323, p = 0.9766), virus x session block (F(7, 104) = 0.6384, p = 0.7232), or virus x tone duration (F (1, 104) = 0.07523, p = 0.7844) interactions were found. D Mean lever press rate during peak trials in the final five sessions of the 24-s peak interval task. E–G Mean best-fitting parameters (derived from fitting to Gaussian function) for peak location (timing accuracy), peak width (timing precision), or peak height (peak response rate). No significant differences were observed in any of these parameters using unpaired t tests.