Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 18;14:4285. doi: 10.1038/s41467-023-39930-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Venn diagram comparison from RNA-Seq data analysis of miR408_OX and miR408_cr poplars. b KEGG pathway enrichment of DEGs that are down-regulated in miR408_OX poplars. c An interaction network of five predicated LACs (LAC19, LAC25, LAC32, LAC47 and LAC55) with miR408 and other miRNAs. The thickness of the green line represents the strength of the interaction. d qRT-PCR showing the relative transcript levels of LAC19, LAC25 and LAC32 with high score using psRNAtarget in WT and miR408_OX and knockout plants. Values are means ± SD (All P-values are from two-tailed Student’s t-tests., n represents 3 trees sampled respectively from miR408_OX #1, miR408_OX #6, miR408_cr #8 and miR408_cr #20). e–g 5’RACE assays showed the cleavage site of the LAC19 (e), LAC25 (f) and LAC32 (g). The red lines in exon 2 show the target sites, and the black arrows show the detailed miR408-guided cleavage positions. h, i Localization of LACs and Pag-miR408 using FISH method. Specific probes were designed to label miR408, LAC19 and LAC25 as green, pink and red, respectively. Lignin autofluorescence is displayed in blue. The merged images showed spatial correspondence between miR408 and LAC19 and LAC25. In young stem (h), miR408 was expressed in all the tissues, while LAC25 was highly expressed in xylem and epidermal cells, and slightly expressed in phloem cells; LAC19 was only expressed in the epidermis and parenchyma cells. In mature stem (i), miR408 was expressed in all the tissues, while LAC25 was highly expressed in xylem, phloem fiber and cortical cells; LAC19 was expressed in cortical and parenchyma cells, but not in phloem fiber or xylem cells. Scale bar, 20 μm. Three samples each were analyzed with similar results. The dashed box represents phloem fibers. Arrows indicate the epidermis. Boxes in h and i represent the enlarged area of cambium and cortex, respectively. Source data are provided as a Source Data file.